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微操作系统技术允许分析骨架调节器的形态发生动力学和营业额
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
DOI:

12:52 min

May 12, 2018

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Chapters

  • 00:05Title
  • 00:50Treatment of Cells, Transfection, and Seeding onto Coverslips
  • 02:54Assembly of Microscopy Imaging Chamber
  • 03:43Microinjection Procedure
  • 05:02FRAP Procedure
  • 05:32Photoactivation Procedure
  • 06:26Data Analysis
  • 09:42Results: Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
  • 11:27Conclusion

Summary

Automatic Translation

我们描述了微和 photomanipulation 技术, 如酶和 photoactivation 如何能够确定运动参数和蛋白质在迁移细胞内的时空动力学。实验读数包括亚细胞动力学和运动调节器的更替或底层肌动蛋白骨架。

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