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人子宫内膜基质细胞的体外分离蜕膜化
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
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人子宫内膜基质细胞的体外分离蜕膜化

DOI:
10.3791/57684-v

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14:55 min

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September 01, 2018

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, , ,
1Department of Obstetrics and Gynecology, Center for Reproductive Health Sciences,Washington University School of Medicine

Chapters

  • 00:00Title
  • 00:56Human Endometrial Stromal Cell Isolation
  • 06:47Human Endometrial Stromal Cell siRNA Transfection
  • 10:06Human Endometrial Stromal Cell In Vitro Decidualization
  • 12:07Results: Decidualization of Human Endometrial Stromal Cells
  • 14:29Conclusion

Summary

Automatic Translation

Automatic Translation

本研究为人子宫内膜间质细胞的分离和培养进行体外蜕膜化试验提供了一个验证和优化的程序。此外, 本研究还提供了一种利用 siRNAs 在人子宫内膜基质细胞中有效地击倒特定基因的方法。

Tags

Human Endometrial Stromal CellsIn Vitro DecidualizationReproductive MedicineDecidualization ProtocolGene KnockdownProlactinInsulin-like Growth Factor Binding Protein OneHBSFHES Isolation MediaDNase-1Collagenase DigestionCell StrainerCell Culture

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