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Gør konjugation-induceret fluorescerende pegyleret viruslignende partikler af Dibromomaleimide-disulfid kemi
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Bioengineering
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JoVE Journal Bioengineering
Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry

Gør konjugation-induceret fluorescerende pegyleret viruslignende partikler af Dibromomaleimide-disulfid kemi

DOI:
10.3791/57712-v

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10:18 min

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May 27, 2018

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, , ,
1Department of Chemistry & Biochemistry,University of Texas at Dallas, 2Undergraduate Biology,University of Texas at Dallas, 3Undergraduate Healthcare Studies,University of Texas at Dallas, 4Departments of Chemistry & Biochemistry and Biomedical Engineering,University of Texas at Dallas

Chapters

  • 00:05Title
  • 00:41Expression of Qβ
  • 02:51Purification of Qβ
  • 05:51Conjugating DB Compounds on Qβ
  • 08:06Results: Qβ-PEG Can Be Used as a Fluorescence Probe Inside Cells
  • 09:35Conclusion

Summary

Automatic Translation

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Vi præsenterer her, en procedure for at fluorescently functionalize disulfider på Qβ VLP med dibromomaleimide. Vi beskriver Qβ udtryk og rensning, syntesen af dibromomaleimide-functionalized molekyler og konjugation reaktionen mellem dibromomaleimide og Qβ. Den resulterende gul fluorescerende konjugeret partikel kan bruges som et fluorescens sonde inde i cellerne.

Tags

PEGylated Virus-like ParticlesDibromomaleimide-disulfide ChemistryBio-conjugationFluorescent FunctionalizationQBeta BacteriophageE. Coli BL21 ExpressionIPTG InductionCell LysisProtein Purification

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