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Journal
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Biochemistry
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マッピング代謝: 監視組織で直接乳酸脱水素酵素活性
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
Please note that all translations are automatically generated.
Click here for the English version.
マッピング代謝: 監視組織で直接乳酸脱水素酵素活性
DOI:
10.3791/57760-v
•
06:18 min
•
June 21, 2018
•
David Jelinek
2
,
Aimee Flores
3
,
Melanie Uebelhoer
,
Vincent Pasque
,
Kathrin Plath
3
,
M. Luisa Iruela-Arispe
3
,
Heather R. Christofk
3
,
William E. Lowry
3
,
Hilary A. Coller
2,3
1
Department of Molecular, Cell and Developmental Biology
,
UCLA
,
2
Department of Biological Chemistry
,
David Geffen School of Medicine
,
3
Molecular Biology Institute Interdepartmental Program
,
UCLA
Chapters
00:04
Title
00:31
Generate Slides of Frozen Mouse Skin
01:53
Slides and Staining Solution Preparation
02:54
Staining and Imaging
04:08
Results: Representative Results of Lactate Dehydrogenase Staining
05:43
Conclusion
Summary
Automatic Translation
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Automatic Translation
Nicotinatmide アデニン酸 (NAD(P)H) + H + 組織サンプルで直接生成酵素の酵素活性の分布をマッピングするためのプロトコルについて述べます。
Tags
Lactate Dehydrogenase
Enzyme Activity
Cryomold
Cryosectioning
Histochemical Staining
NAD
PMS
Nitrotetrazolium Blue Chloride
Lactate
Enzyme Localization
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