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Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart
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Chapters
- 00:04Title
- 01:082-D Image Acquisition
- 02:382-D to 3-D Conversion
- 03:403-D to 4-D Conversion
- 04:41Results: Ventricular Morphologies of Genetically Manipulated Zebrafish
- 06:02Conclusion
Here, we present a protocol to visualize developing hearts in zebrafish in 4-Dimensions (4-D). 4-D imaging, via light-sheet fluorescence microscopy (LSFM), takes 3-Dimensional (3-D) images over time, to reconstruct developing hearts. We show qualitatively and quantitatively that shear stress activates endocardial Notch signaling during chamber development, which promotes cardiac trabeculation.
