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Journal
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Genetics
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单分子荧光
原位
杂交 (smFISH) 在发芽酵母营养生长和减数分裂中的分析
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Single Molecule Fluorescence
In Situ
Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
Please note that all translations are automatically generated.
Click here for the English version.
单分子荧光
原位
杂交 (smFISH) 在发芽酵母营养生长和减数分裂中的分析
DOI:
10.3791/57774-v
•
09:28 min
•
May 25, 2018
•
Jingxun Chen
,
David McSwiggen
,
Elçin Ünal
1
Department of Molecular and Cell Biology, Barker Hall
,
University of California, Berkeley
,
2
Department of Molecular and Cell Biology, Li Ka Shing Center
,
University of California, Berkeley
Chapters
00:05
Title
00:40
Sample Fixation and Digestion
03:50
Hybridization
05:03
Washing and Preparing Cells for Imaging
07:03
Results: smFISH Detects Individual mRNA Isoforms of
NDC80
in Single Cells
08:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该单分子荧光
原位
杂交协议的优化, 以量化的 RNA 分子在萌芽期酵母在营养生长和减数分裂。
Tags
Single Molecule RNA
SmFISH
Budding Yeast
Vegetative Growth
Meiosis
Gene Expression
Subcellular RNA Localization
Formaldehyde Fixation
Vanadyl Ribonucleoside Complexes
Buffer B
Zymolyase
Digestion
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