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Journal
/
Immunology and Infection
/
猕猴免疫缺陷病毒感染 CD4
+
T 细胞单细胞定量的 mRNA 和表面蛋白的表达
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4
+
T Cells Isolated from Rhesus macaques
Please note that all translations are automatically generated.
Click here for the English version.
猕猴免疫缺陷病毒感染 CD4
+
T 细胞单细胞定量的 mRNA 和表面蛋白的表达
DOI:
10.3791/57776-v
•
13:13 min
•
September 25, 2018
•
Andrey Tokarev
,
Matthew Creegan
,
Michael A. Eller
,
Mario Roederer
,
Diane L. Bolton
1
US Military HIV Research Program, Henry M. Jackson Foundation
,
Walter Reed Army Institute of Research
,
2
Vaccine Research Center, NIAID
,
NIH
Chapters
00:04
Title
00:46
Gene Expression Assay Preparation and Surface Staining of Viable Cells
03:18
Prepare Cell Collection Plates, Perform FACS Sort, and Generate cDNA
05:17
Multiplex qPCR on the Biomark Platform
11:26
Results: Single-cell Quantitation of mRNA and Surface Protein Expression
12:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述的方法, 以定量的表达96基因和18表面蛋白的单细胞
前
体, 允许鉴定差异表达基因和蛋白质的病毒感染细胞相对未感染的细胞。我们采用这种方法来研究与恒河猴分离的 SIV 感染的 CD4
+
T 细胞。
Tags
Single-cell Quantitation
MRNA
Surface Protein
Simian Immunodeficiency Virus
CD4+ T Cells
Rhesus Macaques
Host-pathogen Interactions
Cell Sorting
Flow Cytometry
Gene Expression Assays
Surface Staining
Cell Suspension
Cell Viability
Antibody Cocktail
Cell Strainer
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