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Encellede kvantitering af mRNA og overflade Protein udtryk i Simian immundefektvirus-inficerede CD4+ T celler isoleret fra Rhesus makakaber
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
DOI:

13:13 min

September 25, 2018

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Chapters

  • 00:04Title
  • 00:46Gene Expression Assay Preparation and Surface Staining of Viable Cells
  • 03:18Prepare Cell Collection Plates, Perform FACS Sort, and Generate cDNA
  • 05:17Multiplex qPCR on the Biomark Platform
  • 11:26Results: Single-cell Quantitation of mRNA and Surface Protein Expression
  • 12:56Conclusion

Summary

Automatic Translation

Beskrevet er en metode til at kvantificere udtryk for 96 gener og 18 overflade proteiner af enkelt celler ex vivo, giver mulighed for identifikation af varierende udtrykte gener og proteiner i virus-inficerede celler i forhold til ikke-inficerede celler. Vi anvender tilgang til undersøgelse SIV-inficerede CD4+ T celler isoleret fra rhesus makakaber.

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