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Journal
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Developmental Biology
/
斑马鱼原代细胞的培养与转染
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Culture and Transfection of Zebrafish Primary Cells
Please note that all translations are automatically generated.
Click here for the English version.
斑马鱼原代细胞的培养与转染
DOI:
10.3791/57872-v
•
08:51 min
•
August 17, 2018
•
Giulio Russo
2
,
Franziska Lehne
,
Sol M. Pose Méndez
,
Stefan Dübel
,
Reinhard W. Köster
,
Wiebke A. Sassen
1
Division of Cellular and Molecular Neurobiology, Zoological Institute
,
Braunschweig University of Technology
,
2
Department of Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics
,
Braunschweig University of Technology
Chapters
00:04
Title
00:39
Preparation of Primary Cells from Zebrafish Embryos
01:54
Preparation of Reusable Poly-L-lysine-coated Glass Bottom Dishes
03:26
Preparation and Plating of Primary Cells
06:02
Transfection of Primary Cells with Plasmid DNA
06:57
Results: Live Cell Imaging of Zebrafish Primary Cells
08:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个有效的和易于使用的协议, 以制备斑马鱼胚胎的原细胞培养的转染和活细胞成像, 以及一个协议, 以准备初级细胞从成年斑马鱼的大脑。
Tags
Zebrafish
Primary Cells
Cell Culture
Transfection
Intercellular Dynamics
Cell Lines
Embryos
Chorion Removal
Glass-bottom Dishes
Poly-L-lysine Coating
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