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由亲炎细胞因子的内脑注射诱导的广度脑皮质脱叶的鼠模型
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines
Please note that all translations are automatically generated.
Click here for the English version.
由亲炎细胞因子的内脑注射诱导的广度脑皮质脱叶的鼠模型
DOI:
10.3791/57879-v
•
09:46 min
•
September 21, 2021
•
Muammer Üçal*
1
,
Michaela Tanja Haindl*
2
,
Milena Z. Adzemovic
,
Manuel Zeitelhofer
,
Ute Schaefer
,
Franz Fazekas
,
Sonja Hochmeister
1
Research Unit of Experimental Neurotraumatology, Department of Neurosurgery
,
Medical University Graz
,
2
Department of Neurology
,
Medical University Graz
,
3
Centre for Molecular Medicine, Department of Clinical Neuroscience
,
Karolinska Institutet
,
4
Division of Vascular Biology, Department of Medical Biochemistry and Biophysics
,
Karolinska Institutet
Chapters
00:05
Introduction
01:15
Catheter Implantation
04:39
Immunization
05:57
Intracerebral Cytokine Injection
07:16
Results: PLP Immunoreactivity in Coronal Brain Sections
08:52
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里提出的协议允许成年雄性黑阿古提大鼠的皮质半球广泛脱皮。该方法包括导管的内腔植入、骨髓寡细胞糖蛋白的亚临床免疫,以及通过植入导管对促炎细胞因子混合物的内腔注射。
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Rat
Cerebral Cortex
Demyelination
Pro-inflammatory Cytokines
Multiple Sclerosis
Catheter Implantation
Stereotactic Surgery
Cortical Inflammation
Gray Matter Demyelination
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