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JoVE Journal Genetics
Weefsel-specifieke miRNA expressie profilering in muis hart secties gebruiken In Situ hybridisatie
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Weefsel-specifieke miRNA expressie profilering in muis hart secties gebruiken In Situ hybridisatie

Article DOI: 10.3791/57920-v • 08:22 min • September 15th, 2018
September 15th, 2018 •
1, 2, 3
1Department of Cell and Developmental Biology, University College London, 2Yale Cardiovascular Research Group, Section of Cardiovascular Medicine, Yale School of Medicine, 3Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Yale School of Medicine

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Micro-RNAs (miRNAs) zijn korte en zeer homologe RNA-sequenties, post-transcriptional regulatoren van messenger RNAs (mRNAs) bijeenkomen. Huidige miRNA detectiemethoden verschillen in gevoeligheid en specificiteit. We beschrijven een protocol dat in situ hybridisatie en immunokleuring voor gelijktijdige detectie van miRNA en eiwit moleculen op muis hart weefselsecties combineert.

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Genetica kwestie 139 micro-RNA expressie profilering LNA sonde in situ hybridisatie immunokleuring hypertrofie van het hart
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