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Bukspyttkjertelen Holme innebygging parafinsnitt
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JoVE Journal Biology
Pancreatic Islet Embedding for Paraffin Sections

Bukspyttkjertelen Holme innebygging parafinsnitt

DOI:
10.3791/57931-v

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09:09 min

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June 29, 2018

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, , , ,
1Diabetes Center of Excellence,UMass Medical School, 2Department of Medicine,Saint Vincent Hospital, 3Department of Pathology, Morphology Core,UMass Medical School

Chapters

  • 00:04Title
  • 00:35Islet Fixation
  • 02:06Preparation of Islet Disc
  • 05:55Results: Paraffin Section Analysis
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

Ex vivo bukspyttkjertelen Holme studier er viktig for diabetes forskning. Eksisterende teknikker å studere kulturperler holmer i sin opprinnelige 3-dimensjonale arkitektur er tidkrevende, ineffektive og sjelden brukt. Dette verket beskriver en ny, enkel og effektiv metode for å generere høy kvalitet parafinsnitt av hele kulturperler holmer.

Tags

Pancreatic IsletParaffin SectionsEmbedding MethodIslet EquivalentsPBS WashFixativeAgarose Blue BeadsMicropipette TipCentrifugation

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