A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:40Cell Seeding and Treatment
- 02:46Cell Harvest and Preparation for Electrodisruption
- 03:53Plasma Membrane Electrodisruption and Separation of Sedimentable- and Total Cell Fractions
- 05:50LDH Extraction and Measurement of LDH Enzymatic Activity
- 06:55Results: Monitoring Bulk Autophagic Sequestration Activity in Mammalian Cells
- 08:43Conclusion
Aquí se describe un protocolo simple y bien validado para medir la actividad de autofagia secuestro a granel en células de mamíferos. El método se basa en cuantificar la proporción de lactato deshidrogenasa (LDH) en fracciones celulares de sedimentables en comparación con los niveles de LDH celulares total.
