Journal
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Maya Overexpression çiftleşme tabanlı Kütüphane tarama
JoVE Journal
Genetics
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JoVE Journal Genetics
Mating-based Overexpression Library Screening in Yeast
DOI:

11:39 min

July 06, 2018

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Chapters

  • 00:04Title
  • 00:39Yeast Transformation in 96-well Format
  • 05:48Mating Between Cells Containing Library Genes and Query Yeast
  • 08:20Spotting Assay
  • 09:12Results: Successful Screening for Genes that Modify FUS Toxicity
  • 10:44Conclusion

Summary

Automatic Translation

Bu makalede bir dizilmiş plazmid kütüphaneyi kullanan mayası overexpression tarama kolaylaştırmak için bir çiftleşme tabanlı yöntem sunuyor.

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