Journal
/
Neuroscience
/
果蝇幼虫和成年脑的代谢分析
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains
Please note that all translations are automatically generated. Click here for the English version.

果蝇幼虫和成年脑的代谢分析

DOI:
10.3791/58007-v

•

07:06 min

•

August 07, 2018

•

, , , ,
1Department of Biology,Providence College

Chapters

  • 00:04Title
  • 00:40Preparation of the Micro-tissue Restraints
  • 01:23Dissection of the Drosophila melanogaster Larval Brains
  • 02:44Addition of the Dissected Brains and Micro-tissue Restraints to the 96-well Metabolic Assay Cell Plate
  • 04:52Addition of the Cell Plate to the Metabolic Analyzer and the Post-measurement Analysis
  • 05:37Results: The OCR and ECAR Measurements in Adult and Larval Drosophila melanogaster Brains
  • 06:36Conclusion

Summary

Automatic Translation

Automatic Translation

本文提出了一种测定果蝇幼虫和成年脑中氧耗量和细胞外酸化的方法。代谢分析仪是利用一个适应和优化的协议。微组织约束是本协议的重要组成部分, 专门为其在分析中的应用而设计和创建。

Tags

Keywords: Drosophila MelanogasterLarval BrainAdult BrainMetabolic AnalysisMetabolic ReprogrammingGlial CellsDietBehaviorImaginal DiscsC. ElegansDissection96-well Metabolic Assay PlateAssay MediaPBS

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58007?article_type=v

幼虫和成人测绘和增强子陷阱Flippase表达中的应用果蝇中枢神经系统
check_url/58007?article_type=v

形态分析果蝇幼虫外周感觉神经元树突和轴突使用遗传马赛克
check_url/58007?article_type=v

前全体体内培养，发展的果蝇大脑
check_url/58007?article_type=v

幼虫，蛹和成人的解剖和免疫组化<em>果蝇</em>视网膜
check_url/58007?article_type=v

在幼虫馈电电路的功能分析果蝇</i
check_url/58007?article_type=v

的实时成像<em>果蝇</em>幼虫神经母细胞
check_url/58007?article_type=v

收购高品质的数字视频<em>果蝇</em>从横向角度看幼虫及成虫行为
check_url/58007?article_type=v

一个简单的一步解剖协议成人全安装准备<em>果蝇</em>脑
check_url/58007?article_type=v

分析突触调制<em>果蝇</em>曝光时间延长后，光光感受器
check_url/58007?article_type=v

斑马鱼脑幼虫神经元、巨噬细胞和小胶质的分离和 RNA 提取

Read Article