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哺乳动物细胞系的厌氧生长与维持
JoVE Journal
Biology
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JoVE Journal
Biology
Anaerobic Growth and Maintenance of Mammalian Cell Lines
Please note that all translations are automatically generated.
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哺乳动物细胞系的厌氧生长与维持
DOI:
10.3791/58049-v
•
07:15 min
•
July 21, 2018
•
Balbina J. Plotkin
,
Ira M. Sigar
,
Julie A. Swartzendruber
,
Amber Kaminski
1
Department of Microbiology and Immunology
,
Midwestern University
Chapters
00:04
Title
01:11
Prepare Media for Anaerobic Culture of Various Mammalian Cell Lines
02:14
Anoxic Cultivation of Various Mammalian Cell Lines
04:33
Assessment of Phenotypic Cell Differentiation by Microscopy of Anaerobically Cultured Cells
05:10
Results: Cell Viability for Long-term Cultivation Dependent on Initial Seeding Confluence
05:57
Conclusion
Summary
Automatic Translation
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在这里, 我们描述了一种新的方法, 使厌氧长期培养已建立的细胞系。测试的最大生存时间是17天。该方法适用于细胞毒性药物的检测和 anoxically 复制细胞的生理学研究。
Tags
Anaerobic Growth
Mammalian Cell Lines
Anoxic Cultivation
Oxygen Toxicity
Anaerobic Respiration
Metabolism
Stem Cell Cultivation
Oxygen Contamination
Nitrite
DMEM
Vacuum
Anaerobic Chamber
Oxygen Probe
Trypsin
Cell Counting
Cell Viability
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