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Chapters
- 00:04Title
- 01:11Prepare Media for Anaerobic Culture of Various Mammalian Cell Lines
- 02:14Anoxic Cultivation of Various Mammalian Cell Lines
- 04:33Assessment of Phenotypic Cell Differentiation by Microscopy of Anaerobically Cultured Cells
- 05:10Results: Cell Viability for Long-term Cultivation Dependent on Initial Seeding Confluence
- 05:57Conclusion
Her, beskriver vi en ny metode, der giver mulighed for anaerobe langsigtede dyrkning af etablerede cellelinier. Den maksimale overlevelsestid, der blev testet er 17 dage. Denne metode er velegnet til afprøvning af cytotoksiske agenter og udforske fysiologi anoxically kopiere celler.
