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単一細胞の蛍光顕微鏡による Efferocytosis の定量化
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
DOI:

06:15 min

August 18, 2018

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Chapters

  • 00:04Title
  • 00:50Quantifying Efferocytic Uptake and Dynamics Using a Fixed Cell Efferocytosis Assay and Inside-out Staining
  • 05:14Results: Overnight Culture of Jurkat Cells with Staurosporine Results in Apoptosis of Greater than 95% of Cells
  • 05:36Conclusion

Summary

Automatic Translation

Efferocytosis、アポトーシス細胞の貪食除去は恒常性を維持するために必要な受容体と認識、キャニスター、アポトーシス細胞の内面化を可能にするシグナル伝達経路によって促進されます。ここで、efferocytosis の定量化と efferocytic シグナル伝達経路の活性の蛍光顕微鏡のプロトコルを提案する.

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