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Quantification of Efferocytosis by Single-cell Fluorescence Microscopy
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

DOI:
10.3791/58149-v

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06:15 min

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August 18, 2018

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, , ,
1Department of Microbiology and Immunology and the Center for Human Immunology,University of Western Ontario, 2Institute of Infection and Global Health,University of Liverpool, 3Department of Respiratory Research,Aintree University Hospital NHS Foundation Trust

Chapters

  • 00:04Title
  • 00:50Quantifying Efferocytic Uptake and Dynamics Using a Fixed Cell Efferocytosis Assay and Inside-out Staining
  • 05:14Results: Overnight Culture of Jurkat Cells with Staurosporine Results in Apoptosis of Greater than 95% of Cells
  • 05:36Conclusion

Summary

Automatic Translation

Automatic Translation

Efferocytosis, the phagocytic removal of apoptotic cells, is required to maintain homeostasis and is facilitated by receptors and signaling pathways that allow for the recognition, engulfment, and internalization of apoptotic cells. Herein, we present a fluorescence microscopy protocol for the quantification of efferocytosis and the activity of efferocytic signaling pathways.

Tags

Keywords: EfferocytosisSingle-cell Fluorescence MicroscopyApoptotic Cell UptakeMacrophagesJurkat CellsNHS-BiotinCell-tracking DyeFITC-conjugated StreptavidinQuantificationMicroscope Optimization

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