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Kvantificering af Efferocytosis af encellede Fluorescens mikroskopi
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Chapters
- 00:04Title
- 00:50Quantifying Efferocytic Uptake and Dynamics Using a Fixed Cell Efferocytosis Assay and Inside-out Staining
- 05:14Results: Overnight Culture of Jurkat Cells with Staurosporine Results in Apoptosis of Greater than 95% of Cells
- 05:36Conclusion
Efferocytosis, fagocyterende fjernelse af apoptotiske celler, er forpligtet til at opretholde homøostase og lettes ved receptorer og signalering veje, der giver mulighed for anerkendelse, engulfment og internalisering af apoptotiske celler. Heri, præsenterer vi en Fluorescens mikroskopi protokol til kvantificering af efferocytosis og aktivitet af efferocytic signaling veje.
