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Forbigående udtryk i Nicotiana Benthamiana efterlader for Triterpene produktion på en forberedende skala
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
DOI:

08:56 min

August 16, 2018

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Chapters

  • 00:04Title
  • 00:33Prepare Infiltration Suspension
  • 02:11Perform Vacuum Infiltration
  • 03:56Growth and Harvest of the Infiltrated Leaves and Pressurized Solvent Extraction
  • 05:38Basic Ion Exchange Resin Treatment for Removal of Chlorophylls
  • 06:58Results: Infiltration Coverage and Isolated Triterpene Product
  • 07:49Conclusion

Summary

Automatic Translation

Forbigående Heterolog ekspression af biosyntetiske enzymer i Nicotiana benthamiana blade kan aflede endogene forsyninger af 2,3-oxidosqualene mod produktion af nye værdifulde triterpene produkter. Heri er beskrevet en detaljeret protokol for hurtig (5 dage) præparativ-skala produktion af Triterpener og analoger udnytte denne kraftfulde plante-baserede platform.

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