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Developmental Biology
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从人胚胎干细胞中提取合适的视网膜色素上皮组织的工程移植
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
从人胚胎干细胞中提取合适的视网膜色素上皮组织的工程移植
DOI:
10.3791/58216-v
•
07:48 min
•
September 06, 2018
•
Karim Ben M’Barek
2,3
,
Walter Habeler
2,3
,
Alexandra Plancheron
2,3
,
Mohamed Jarraya
,
Olivier Goureau
,
Christelle Monville
2
1
U861, I-Stem, Association Française contre les Myopathies (AFM)
,
Institut National de la Santé et de la Recherche Médicale (INSERM)
,
2
U861, I-Stem, Association Française contre les Myopathies (AFM)
,
Université Evry Val-d’Essonne (UEVE)
,
3
I-Stem, Association Française contre les Myopathies (AFM)
,
Centre pour L’Etude des Cellules Souches (CECS)
,
4
Banque de tissus humain, Hôpital Saint Louis, Assistance Publique – Hôpitaux de Paris (AP-HP)
,
5
Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012
Chapters
00:04
Title
00:36
Human Amniotic Membrane (hAM) Thermolysin Treatment and Fixation
02:27
Retinal Pigment Epithelium (RPE) Cell Seeding
03:33
RPE Patch Transplantation Preparation
05:58
Results: Representative RPE-seeded hAM
06:38
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了一种由人类羊膜顶部培养的人多能干细胞的视网膜色素上皮细胞组成的视网膜组织的方法及其在动物模型中的移植准备。
Tags
Retinal Pigment Epithelium
Human Embryonic Stem Cells
Eye Implantation
RPE Cells
Amniotic Membrane
Nylon Scaffold
Thermolysin
Culture Insert
Cell Seeding
Cell Culture
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