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Journal
/
Biochemistry
/
无细胞
爪
卵萃取物微乳液细胞周期振荡的重建
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free
Xenopus
Egg Extracts
Please note that all translations are automatically generated.
Click here for the English version.
无细胞
爪
卵萃取物微乳液细胞周期振荡的重建
DOI:
10.3791/58240-v
•
06:31 min
•
September 27, 2018
•
Ye Guan
2
,
Shiyuan Wang
,
Minjun Jin
,
Haotian Xu
,
Qiong Yang
4
1
Department of Biophysics
,
University of Michigan, Ann Arbor
,
2
Department of Chemistry
,
University of Michigan, Ann Arbor
,
3
Department of Computer Science
,
Wayne State University
,
4
Department of Physics
,
University of Michigan, Ann Arbor
Chapters
00:04
Title
00:41
Preparation of Cycling
Xenopus
Extracts
03:39
Droplet Generation and Imaging
05:19
Results: Cell-cycle System Capable of Reconstructing Mitotic Oscillator of Cdk1 and APC/C
05:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提出了在油中微乳液中对
爪蟾
卵提取物进行单细胞水平的
体外
自持续有丝分裂振荡的生成方法。
Tags
Cell-cycle Oscillations
Xenopus Egg Extracts
Microemulsions
Cell-free System
Mitotic Network
Cell Cycle Clock
High-throughput
Quantitative Manipulation
Cell Activation
Cell Lysis
Cell Extract Preparation
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