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Antigene Liposomer for Generation af sygdoms-specifikke antistoffer
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1Janssen R&D, 2Department of Microbiology and Immunology,University of North Carolina, 3UNC Food Allergy Initiative,University of North Carolina, 4Department of Pediatrics,University of North Carolina, 5Department of Chemistry,University of Alberta, 6Department of Molecular Medicine,Scripps Research Institute, 7Department of Medical Microbiology and Immunology,University of Alberta
Chapters
- 00:04Title
- 01:13Protein Antigen-PEGylated Lipid Conjugation
- 02:57Liposome Extrusion
- 04:24Calcium Flux Monitoring of Antigenic Liposome-induced B Cell Activation
- 06:33Mouse Peanut Sensitization and Ara h 2 (Ah2) Antigenic Liposome Challenge
- 08:26Results: Representative Allergic Response Analyses
- 09:39Conclusion
Beskrevet er forberedelsen af antigene liposomal nanopartikler og deres anvendelse i stimulerende B-celle aktivering i in vitro og i vivo. Sammenhængende og robust antistof svar førte til udviklingen af en ny jordnøddeallergi model. Protokol til at generere antigene Liposomer kan udvides til at omfatte forskellige antigener og immunisering modeller.
