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Journal
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Immunology and Infection
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抗原脂质体用于生成特定于疾病的抗体
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Antigenic Liposomes for Generation of Disease-specific Antibodies
Please note that all translations are automatically generated.
Click here for the English version.
抗原脂质体用于生成特定于疾病的抗体
DOI:
10.3791/58285-v
•
10:31 min
•
October 25, 2018
•
Kyle J. Bednar*
1
,
Lakeya Hardy*
2,3
,
Johanna Smeekens*
3,4
,
Dharmendra Raghuwanshi
,
Shiteng Duan
,
Mike D. Kulis
4
,
Matthew S. Macauley
7
1
Janssen R&D
,
2
Department of Microbiology and Immunology
,
University of North Carolina
,
3
UNC Food Allergy Initiative
,
University of North Carolina
,
4
Department of Pediatrics
,
University of North Carolina
,
5
Department of Chemistry
,
University of Alberta
,
6
Department of Molecular Medicine
,
Scripps Research Institute
,
7
Department of Medical Microbiology and Immunology
,
University of Alberta
Chapters
00:04
Title
01:13
Protein Antigen-PEGylated Lipid Conjugation
02:57
Liposome Extrusion
04:24
Calcium Flux Monitoring of Antigenic Liposome-induced B Cell Activation
06:33
Mouse Peanut Sensitization and Ara h 2 (Ah2) Antigenic Liposome Challenge
08:26
Results: Representative Allergic Response Analyses
09:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述了抗原脂质体纳米粒子的制备及其在
体外
和
体内
刺激 B 细胞活化的作用。一致和稳健的抗体反应导致了一个新的花生过敏模型的发展。抗原脂质体的生成协议可以扩展到不同的抗原和免疫模型。
Tags
Antigenic Liposomes
Disease-specific Antibodies
Immunology
Allergy Mouse Model
Autoimmunity
Liposomal Nanoparticles
Heterobifunctional Crosslinker
SPDP
Dithiothreitol
DSPE-PEG2000-Maleimide
Lipid-linked Protein
Lyophilization
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