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抗原脂质体用于生成特定于疾病的抗体
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Antigenic Liposomes for Generation of Disease-specific Antibodies
DOI:

10:31 min

October 25, 2018

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Chapters

  • 00:04Title
  • 01:13Protein Antigen-PEGylated Lipid Conjugation
  • 02:57Liposome Extrusion
  • 04:24Calcium Flux Monitoring of Antigenic Liposome-induced B Cell Activation
  • 06:33Mouse Peanut Sensitization and Ara h 2 (Ah2) Antigenic Liposome Challenge
  • 08:26Results: Representative Allergic Response Analyses
  • 09:39Conclusion

Summary

Automatic Translation

描述了抗原脂质体纳米粒子的制备及其在体外体内刺激 B 细胞活化的作用。一致和稳健的抗体反应导致了一个新的花生过敏模型的发展。抗原脂质体的生成协议可以扩展到不同的抗原和免疫模型。

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