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Journal
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Immunology and Infection
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双光子活体显微镜感病毒感染时气管黏膜的影像细胞相互作用
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
双光子活体显微镜感病毒感染时气管黏膜的影像细胞相互作用
DOI:
10.3791/58355-v
•
08:01 min
•
August 17, 2018
•
Miguel Palomino-Segura*
1,2
,
Tommaso Virgilio*
1,2
,
Diego Morone
,
Diego U. Pizzagalli
3
,
Santiago F. Gonzalez
1
Faculty of Biomedical Sciences, Institute for Research in Biomedicine
,
Università della Svizzera italiana (USI)
,
2
Graduate School of Cellular and Molecular Sciences, Faculty of Medicine
,
University of Bern
,
3
Institute of Computational Science
,
Università della Svizzera italiana (USI)
Chapters
00:04
Title
00:57
Intranasal Influenza Infection and Neutrophil Isolation and Injection
03:21
Surgical Preparation
05:04
In Vivo
Time Lapse Imaging
06:01
Results: Representative Intravital 4D Imaging and Analyses
07:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
在本研究中, 我们提出了一个协议, 执行双光子活体成像和细胞相互作用分析的小鼠气管粘膜感染后流感病毒。这个协议将是相关的研究人员研究免疫细胞动力学在呼吸道感染。
Tags
Two-photon Intravital Microscopy
Immune Responses
Airborne Pathogens
Neutrophil Interactions
Influenza Infection
CD11c-YFP Mouse
PR8 Inoculate
Bone Marrow
Percoll Gradient
Neutrophil Isolation
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