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Syntes och strukturbestämning av µ-Conotoxin PIIIA isomerer med olika disulfid Connectivities
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities
DOI:

11:44 min

October 02, 2018

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Chapters

  • 00:04Title
  • 00:49Solid-Phase Peptide Synthesis (SPPS), Peptide Cleavage from the Resin, and Purification of the Linear Precursor
  • 03:04Selective Formation of the Disulfide Bonds
  • 04:44Peptide Purification
  • 05:56Peptide Analytics
  • 08:04MS/MS Analysis of Disulfide Connectivity
  • 09:49Results: The Sequential Walk and the Resulting NMR Solution Structure of a Rigid and a Flexible -PIIIa Isomer
  • 10:30Conclusion

Summary

Automatic Translation

Cystein-rika peptider vik distinkta tredimensionella strukturer beroende på deras disulfid anslutning. Riktade syntes av enskilda disulfid isomerer krävs när bufferten oxidation inte leder till önskad disulfid anslutning. Protokollet behandlar selektiv syntesen av 3-disulfid-bundna peptider och deras strukturella analys med hjälp av NMR- och MS/MS studier.

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