Biology
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Изоляция CD4+ Т-клеток и анализ циркулирующих Т-фолликулярная помощник (cTfh) клеток подмножеств из периферической крови с помощью 6-цвет потока цитометрии
Chapters
Summary January 7th, 2019
Please note that all translations are automatically generated.
Click here for the English version.
Здесь, протокол для изоляции и характеристика CD4+ Т-клеток описал подмножеств из периферической крови человека. Очищенный CD4+ Т-клетки анализируются проточной цитометрии для определения пропорции T-фолликулярных клеток помощник подмножеств.
Transcript
Этот метод может помочь продемонстрировать различия между циркулирующими Т-фолликулярными подмножествами клеток-помощников в здоровых и больных состояниях. Основные преимущества этого метода в том, что он быстрый, что образцы могут быть обработаны пакетами, и что клетки остаются в живых для вниз по течению функционального анализа. Этот метод облегчает оценку циркулирующих T-фолликулярных подмножества помощников в качестве биомаркеров для прогрессирования заболевания, поскольку они коррелируют с маркерами клинической тяжести. Помощь в демонстрации этой процедуры будет д-р Люсия Пи
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