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Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
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Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

DOI:
10.3791/60475-v

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07:57 min

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December 26, 2019

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, , , , ,
1Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne,University of Lausanne, 2Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences,Swiss Federal Institute of Technology Lausanne (EPFL), 3Center of Experimental Therapeutics, Department of Oncology,Centre Hospitalier Universitaire Vaudois, 4Hematology Service, Department of Oncology,Centre Hospitalier Universitaire Vaudois (CHUV)

Chapters

  • 00:04Title
  • 01:59Ex Vivo Culture of FACS Sorted Hematopoietic Stem Cells
  • 04:00Measurement of Mitochondrial Mass and Membrane Potential
  • 06:21Results: Mitochondrial Profiles
  • 07:18Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a reliable method to measure mitochondrial mass and membrane potential in ex vivo cultured hematopoietic stem cells and T cells.

Tags

Mitochondrial MassMitochondrial Membrane PotentialFlow CytometryHematopoietic Stem CellsT-cellsMetabolic AssaysBone Marrow TransplantationColony-forming AssaysImmune T CellsVerapamilFACS SortingLKS PopulationCD150CD48Stem Cell Expansion Medium

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