Journal
/
Bioengineering
/
阳离子纳米脂质体的生成, 用于高效输送体外转录信使 rna
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Please note that all translations are automatically generated. Click here for the English version.

阳离子纳米脂质体的生成, 用于高效输送体外转录信使 rna

DOI:
10.3791/58444-v

•

08:29 min

•

February 01, 2019

•

, , , , , , ,
1Department of Thoracic and Cardiovascular Surgery, Clinical Research Laboratory,University Medical Center, 2Atherothrombosis and Vascular Biology,Baker Heart & Diabetes Institute, 3Department of Medicine,Monash University

Chapters

  • 00:04Title
  • 00:48Generation of Cationic Nanoliposomes
  • 02:25Complexation of Synthetic mRNA
  • 03:03Preparation of the Cells for Transfection and Transfection of the Cells
  • 03:54Analysis of Cell Transfection Efficacy Using Flow Cytometry and Fluorescence Microscopy
  • 05:33Results: Analysis of Generated Cationic Nanoliposomes
  • 07:13Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了一个方案的阳离子纳米脂质体的生成, 这是基于干膜法, 并可用于安全和有效的传递体外转录信使 rna。

Tags

Cationic NanoliposomesMRNA DeliveryTransfectionNanoparticleLipid FilmExtrusionNanolipoplexA549 Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

微型涡辅助电穿孔的顺序传递分子

三维全层皮肤的生成等效和自动伤人

眼刺激试验（EIT）使用重构型人角膜上皮样（RHCE）组织模型眼刺激性化学品危险性鉴定

适应静电过程中提供三个独特的环境为一个三层次<em>在体外</em>的气道壁模型

治疗的siRNA递送至CNS使用阳离子和阴离子脂质体

聚电解质复合物肝素结合域成骨生长因子交货

一个<em>体外</em鼠类的椎间盘>器官培养模型

利用 CRISPR-Cas9 系统高效生成和编辑人胰细胞无饲养干细胞

生物活性亲水球状蛋白的纳米脂质体制备与表征

人体神经细胞在体外热的化学和分子分析的生物粒子微阵列

Read Article