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果蝇视觉系统中低丰细胞的纯化
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Genetics
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JoVE Journal
Genetics
Purification of Low-abundant Cells in the Drosophila Visual System
Please note that all translations are automatically generated.
Click here for the English version.
果蝇视觉系统中低丰细胞的纯化
DOI:
10.3791/58474-v
•
07:09 min
•
September 26, 2018
•
Jing Peng
,
Ivan J. Santiago
,
Matthew Y. Pecot
1
Department of Neurobiology
,
Harvard Medical School
Chapters
00:04
Title
00:30
Papain and Proteolytic Enzyme Blend
01:49
Dissections
02:44
Cell Dissociation
05:45
Results: Effective Purification of Low-abundant
Drosophila Cells
06:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了细胞离解协议, 以有效地隔离的细胞在低丰度的果蝇视觉系统通过荧光活化细胞排序 (外地资产管制署)。
Tags
Cell Purification
Drosophila Visual System
Transcriptomic Analysis
Genomic Analysis
Papain
Proteolytic Enzyme
Optic Lobes
Microtube
Dissection
Low-abundant Cells
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