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Optimeret Interferon-gamma ELISpot Assay til foranstaltning T-celle respons i Guinea Gris Model efter vaccinationen
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Optimeret Interferon-gamma ELISpot Assay til foranstaltning T-celle respons i Guinea Gris Model efter vaccinationen

DOI:
10.3791/58595-v

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08:13 min

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January 20, 2019

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, , , , , , , ,
1Inovio Pharmaceuticals, Inc, 2Mycotic and Parasitic Agents and Mycobacteria,Robert Koch Institute

Chapters

  • 00:04Title
  • 00:58Preparation of ELISpot-plates and Cell-stimulation
  • 02:09Blood Sample Processing
  • 03:44Plate Preparation and Cell-plating
  • 04:47Detection of Interferon-gamma Positive Spots
  • 06:34Results: ELISpot Assay Used to Characterize T-cell Responses in Guinea Pigs
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

Udviklingen af interferon-gamma ELISpot assay for marsvin PBMC tillader karakterisering af T-celle svar i denne meget relevante model til at studere infektionssygdomme. Vi har anvendt analyse til måling af T celle svar tilknyttet intradermal levering af DNA-vacciner.

Tags

ELISpot AssayT Cell ResponsesGuinea Pig ModelVaccinationImmunotherapeuticsHumoral ImmunityPeripheral BloodKineticsCapture Anti-guinea Pig Interferon Gamma Antibody VE4Density Gradient MediumPBMCs

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