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H3 ・ H4 ヒストン蛋白質細胞および脳組織におけるアセチル化ヒストンの定量化の浄化
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
Please note that all translations are automatically generated.
Click here for the English version.
H3 ・ H4 ヒストン蛋白質細胞および脳組織におけるアセチル化ヒストンの定量化の浄化
DOI:
10.3791/58648-v
•
09:43 min
•
November 30, 2018
•
Karolina J. Janczura
2
,
Claude-Henry Volmar
2
,
Claes Wahlestedt
2
1
Department of Psychiatry & Behavioral Sciences
,
University of Miami Miller School of Medicine
,
2
Center for Therapeutic Innovation
,
University of Miami Miller School of Medicine
Chapters
00:04
Title
01:06
Preparation of the Sample Extract
02:38
Preparation of the Crude Histone Extract
03:33
Evaluation of the Presence of Histone and/or Non-histone Protein in Histone Extract
04:14
Purification of Core Histones
05:13
Precipitation of Core Histones
07:02
Results: Human Microglial BV2 Histone Purified and Quantification in Wild Type and Transgenic Mouse Brain
09:02
Conclusion
Summary
Automatic Translation
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Automatic Translation
この資料の目的は、ヒストン H3、H4 の効率的な精製と残基のアセチル化ヒストンの定量化に包括的、体系的なガイドを提供することです。
Tags
Histone Proteins
H3
H4
Acetylation
Post-translational Modifications
Purification
Quantification
Cell Extracts
Alzheimer’s Disease
Epigenetic Regulation
Cell Culture
Centrifugation
Buffer Extraction
PH Neutralization
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