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H3 ・ H4 ヒストン蛋白質細胞および脳組織におけるアセチル化ヒストンの定量化の浄化
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
DOI:

09:43 min

November 30, 2018

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Chapters

  • 00:04Title
  • 01:06Preparation of the Sample Extract
  • 02:38Preparation of the Crude Histone Extract
  • 03:33Evaluation of the Presence of Histone and/or Non-histone Protein in Histone Extract
  • 04:14Purification of Core Histones
  • 05:13Precipitation of Core Histones
  • 07:02Results: Human Microglial BV2 Histone Purified and Quantification in Wild Type and Transgenic Mouse Brain
  • 09:02Conclusion

Summary

Automatic Translation

この資料の目的は、ヒストン H3、H4 の効率的な精製と残基のアセチル化ヒストンの定量化に包括的、体系的なガイドを提供することです。

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