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Neuroscience
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h3 和 h4 组蛋白的纯化及细胞和脑组织中乙酰化组蛋白标记的定量
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
Please note that all translations are automatically generated.
Click here for the English version.
h3 和 h4 组蛋白的纯化及细胞和脑组织中乙酰化组蛋白标记的定量
DOI:
10.3791/58648-v
•
09:43 min
•
November 30, 2018
•
Karolina J. Janczura
2
,
Claude-Henry Volmar
2
,
Claes Wahlestedt
2
1
Department of Psychiatry & Behavioral Sciences
,
University of Miami Miller School of Medicine
,
2
Center for Therapeutic Innovation
,
University of Miami Miller School of Medicine
Chapters
00:04
Title
01:06
Preparation of the Sample Extract
02:38
Preparation of the Crude Histone Extract
03:33
Evaluation of the Presence of Histone and/or Non-histone Protein in Histone Extract
04:14
Purification of Core Histones
05:13
Precipitation of Core Histones
07:02
Results: Human Microglial BV2 Histone Purified and Quantification in Wild Type and Transgenic Mouse Brain
09:02
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文的目的是为组蛋白 h3 和 h4 的有效纯化以及乙酰化组蛋白残留量的定量提供全面、系统的指导。
Tags
Histone Proteins
H3
H4
Acetylation
Post-translational Modifications
Purification
Quantification
Cell Extracts
Alzheimer’s Disease
Epigenetic Regulation
Cell Culture
Centrifugation
Buffer Extraction
PH Neutralization
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