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Rensning af H3 og H4 Histon proteiner og kvantificering af acetyleret Histon mærker i celler og hjernevæv
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
DOI:

09:43 min

November 30, 2018

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Chapters

  • 00:04Title
  • 01:06Preparation of the Sample Extract
  • 02:38Preparation of the Crude Histone Extract
  • 03:33Evaluation of the Presence of Histone and/or Non-histone Protein in Histone Extract
  • 04:14Purification of Core Histones
  • 05:13Precipitation of Core Histones
  • 07:02Results: Human Microglial BV2 Histone Purified and Quantification in Wild Type and Transgenic Mouse Brain
  • 09:02Conclusion

Summary

Automatic Translation

Formålet med denne artikel er at give en omfattende, systematisk guide til effektiv rensning af histonerne H3 og H4 og kvantificering af acetyleret Histon restkoncentrationer.

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