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Arıtma H3 ve H4 histon proteinleri ve Acetylated histon işaretleri hücrelerinde ve beyin dokusu miktar
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
DOI:

09:43 min

November 30, 2018

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Chapters

  • 00:04Title
  • 01:06Preparation of the Sample Extract
  • 02:38Preparation of the Crude Histone Extract
  • 03:33Evaluation of the Presence of Histone and/or Non-histone Protein in Histone Extract
  • 04:14Purification of Core Histones
  • 05:13Precipitation of Core Histones
  • 07:02Results: Human Microglial BV2 Histone Purified and Quantification in Wild Type and Transgenic Mouse Brain
  • 09:02Conclusion

Summary

Automatic Translation

Bu makalenin amacı, bir kapsamlı, sistematik rehber histon H3 ve H4 etkili arınma ve miktar acetylated histon kalıntılarının sağlamaktır.

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