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建立过度表达 dr3 的细胞系, 评估抗有丝分裂治疗的凋亡反应
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Cancer Research
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JoVE Journal Cancer Research
Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics
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建立过度表达 dr3 的细胞系, 评估抗有丝分裂治疗的凋亡反应

DOI:
10.3791/58705-v

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12:28 min

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January 11, 2019

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, , ,
1School of Pharmaceutical Sciences,Tsinghua University, 2Department of Radiology,University of California, San Francisco, 3Beijing Advanced Innovation Center for Structural Biology

Chapters

  • 00:04Title
  • 01:03Generation of DR3 Overexpression Cell Lines
  • 05:41Verification of the DR3 Overexpression Cell Line
  • 07:46Assessment of the Apoptotic Response of Cells to Antimitotic Agents
  • 09:51Results: Assessment of the Apoptotic Response to Anti-mitotic Therapeutics
  • 11:15Conclusion

Summary

Automatic Translation

Automatic Translation

建立一个稳定的细胞系, 过度表达一个有兴趣的基因, 研究基因功能, 可以通过稳定的转化后, 选择单一克隆通过逆转录病毒感染后。在这里, 我们表明, ht29-dr3 细胞系产生这种方式阐明的机制, 死亡受体 3 (dr3) 有助于抗肿瘤诱导的细胞凋亡。

Tags

Cell LinesOverexpressingDR3Apoptotic ResponseAnti-mitotic TherapeuticsStable TransfectionRetrovirusFLAG-tagged DR3DR3 AntibodyDR3 Function StudySingle ColonyHomogeneityPurityPlat-A CellsTransfectionViral SuspensionHT29 CellsPolybreneTrypsinizationFBSCentrifugation

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