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Analyse af Shear Flow-induceret Migration af Murine Marginal Zone B-celler In Vitro
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Chapters
- 00:04Title
- 00:43Preparing the 6-chambered Slides for the Flow Experiment
- 01:49Fluidics Unit Preparation
- 03:07Cell Loading
- 04:04Attaching the Fluidics Unit and Imaging the Cells
- 05:28Migration Track Analysis
- 07:02Results: Quantifying Marginal Zone B-cell Shuttling
- 08:00Conclusion
Marginal zone B celler (MZBs) reagere på kraften af shear flow ved re orientere deres overflytningssti op for strømmen. Denne protokol viser, hvordan man registrere og analysere migration ved hjælp af en fluidics enhed, pumpe, mikroskop billedbehandlingssystem og fri software.
