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基因治疗诱导聚化涂层保护 dna 折纸纳米结构
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
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基因治疗诱导聚化涂层保护 dna 折纸纳米结构

DOI:
10.3791/58771-v

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08:30 min

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January 19, 2019

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1Molecular Diagnostics, Centre for Health and Bioresources,AIT Austrian Institute of Technology GmbH

Chapters

  • 00:04Title
  • 01:12Polyplex Formation
  • 02:16Agarose Gel Electrophoresis
  • 03:12Stability of Polyplexes Towards DNase I
  • 04:36Testing the Addressability of Horseradish Peroxidase Enzyme (HRP)-functionalized DNA Origami (HRP-NR) Upon Polycation Coating
  • 05:44Results: Characterization of DNA Origami Polyplexes
  • 07:42Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了一种利用天然阳离子多糖壳聚糖和合成线性聚乙烯胺 (lpei) 涂层保护镁-贫化和富含核酸酶介质中 dna 折纸纳米结构的方案。

Tags

DNA OrigamiNanostructuresGene TherapyPolycation CoatingEnzymatic DegradationCellular UptakeGene DeliveryAgarose Gel Electrophoresis

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