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Bioengineering
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同时进行机电刺激增强细胞心肌成功能的潜能
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Simultaneous Electrical and Mechanical Stimulation to Enhance Cells’ Cardiomyogenic Potential
Please note that all translations are automatically generated.
Click here for the English version.
同时进行机电刺激增强细胞心肌成功能的潜能
DOI:
10.3791/58934-v
•
07:41 min
•
January 18, 2019
•
Aida Llucià-Valldeperas
2
,
Ramon Bragós
,
Antoni Bayés-Genís
4,5,6
1
Insuficiencia Cardiaca y Regeneración Cardiaca (ICREC) Research Program
,
Health Science Research Institute Germans Trias i Pujol
,
2
Amsterdam Universitair Medisch Centrum (UMC), Vrije Universiteit Amsterdam, Pulmonology and Physiology
,
Amsterdam Cardiovascular Sciences
,
3
Electronic and Biomedical Instrumentation Group, Departament d’Enginyeria Electrònica
,
Universitat Politècnica de Catalunya
,
4
Cardiology Service
,
Germans Trias i Pujol University Hospital
,
5
Department of Medicine
,
Universitat Autònoma de Barcelona
,
6
Centro de Investigación Biomédica en Red (CIBER) Cardiovascular
,
Instituto de Salud Carlos III
Chapters
00:04
Title
01:06
Cell Seeding (Day – 1)
02:52
Electromechanical Stimulation Setup (Day 0)
05:55
Results: Gene and Protein Expression of the Main Cardiac Markers after the Electromechanical Stimulation of Cardiac and Subcutaneous ATDPCs
07:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议, 训练细胞群使用电子和机械刺激模拟心脏生理。这种机电刺激增强了治疗细胞的心肌潜能, 是进一步进行细胞治疗、疾病建模和药物筛选的有希望的策略。
Tags
Electromechanical Stimulation
Cell Therapy
Cardiac ATDPCs
PDMS Constructs
Cell Seeding
Cell Culture
Sterile Technique
Centrifugation
Cell Counting
Cell Incubation
Stimulation Unit
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