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Journal
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Immunology and Infection
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atp 利用商业检测和染色法
定量检测淋病奈瑟
菌骨料的抗生素敏感性
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Quantitative Examination of Antibiotic Susceptibility of
Neisseria gonorrhoeae
Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
Please note that all translations are automatically generated.
Click here for the English version.
atp 利用商业检测和染色法
定量检测淋病奈瑟
菌骨料的抗生素敏感性
DOI:
10.3791/58978-v
•
08:04 min
•
February 08, 2019
•
Liang-Chun Wang
,
Jacob Wagner
,
Annabelle Capino
,
Elizabeth Nesbit
,
Wenxia Song
,
Daniel C. Stein
1
Department of Marine Biotechnology and Resources
,
National Sun Yat-Sen University
,
2
Department of Cell Biology and Molecular Genetics
,
University of Maryland
Chapters
00:04
Title
00:47
Neisseria gonorrhoeae
(GC) Strain Maintenance
01:31
GC Aggregate Quantification
03:13
GC Live/Dead Aggregate Analysis
04:26
Image Analysis
05:48
Results: Representative GC Aggregation Survival Before and After Antibiotic Treatment
07:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
采用简单的 atp 测量法和活死染色法对头孢曲松治疗后淋病
奈瑟
菌的生存率进行了定量和可视化。该方案可以扩展到检查任何抗生素的抗菌作用, 并可用于确定细菌生物膜中抗生素的最小抑制浓度。
Tags
Neisseria Gonorrhoeae
Antibiotic Susceptibility
ATP-utilization Assay
Live/dead Staining
Bacterial Aggregates
Ceftriaxone
Microbial Culture
Optical Density
Sonication
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