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Avanceret billedbehandling af lunge Homing humane lymfocytter i en eksperimenterende i Vivo Model af allergisk betændelse baseret på lys-ark mikroskopi
JoVE Journal
Medicine
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JoVE Journal Medicine
Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
DOI:

10:39 min

April 16, 2019

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Chapters

  • 00:04Title
  • 01:02Mouse Allergic Lung Inflammation Induction and Human Peripheral Blood CD4+ T Cell Labeling
  • 02:42CD4+ T Cell Adoptive Transfer and Lung Tissue Preparation
  • 05:26Murine Lung Light-sheet Fluorescence Microscopy
  • 06:43Post-image Processing and Lung-accumulated Human Cell Quantification
  • 08:12Results: Representative Human CD4+ T Cell Lung Accumulation Analysis
  • 09:50Conclusion

Summary

Automatic Translation

Her introducerede protokollen tillader karakterisering af primære humane lymfocytter under in vivo betændelsestilstande homing lunge-kapacitet. Pulmonal infiltration af adoptively overførte human immun celler i en musemodel af allergisk betændelse kan afbildet og kvantificeres ved lys-ark Fluorescens mikroskopi af kemisk ryddet lungevæv.

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