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Chapters
- 00:04Title
- 01:02Mouse Allergic Lung Inflammation Induction and Human Peripheral Blood CD4+ T Cell Labeling
- 02:42CD4+ T Cell Adoptive Transfer and Lung Tissue Preparation
- 05:26Murine Lung Light-sheet Fluorescence Microscopy
- 06:43Post-image Processing and Lung-accumulated Human Cell Quantification
- 08:12Results: Representative Human CD4+ T Cell Lung Accumulation Analysis
- 09:50Conclusion
Her introducerede protokollen tillader karakterisering af primære humane lymfocytter under in vivo betændelsestilstande homing lunge-kapacitet. Pulmonal infiltration af adoptively overførte human immun celler i en musemodel af allergisk betændelse kan afbildet og kvantificeres ved lys-ark Fluorescens mikroskopi af kemisk ryddet lungevæv.
