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JoVE Journal
Biochemistry

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rad51 介导的 dna 链交换反应的实时观测
 
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rad51 介导的 dna 链交换反应的实时观测

Article DOI: 10.3791/59073-v
February 13th, 2019

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Summary February 13th, 2019

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建立了以光谱51介导的 dna 链交换反应为基础的荧光共振能量转移实时观测系统。利用这里提出的协议, 我们能够检测反应中间体的形成及其转化为产物, 同时也分析了反应的酶动力学。

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