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Journal
/
Immunology and Infection
/
一种高通
量志
贺氏菌特异性杀菌试验
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
A High-throughput
Shigella
-specific Bactericidal Assay
Please note that all translations are automatically generated.
Click here for the English version.
一种高通
量志
贺氏菌特异性杀菌试验
DOI:
10.3791/59164-v
•
06:11 min
•
February 27, 2019
•
Hailey P. Weerts
,
Jigui Yu
,
Robert W. Kaminski
,
Moon H. Nahm
1
Department of Enteric Infections, Bacterial Diseases Branch
,
Walter Reed Army Institute of Research
,
2
University of Alabama at Birmingham
Chapters
00:04
Title
00:44
Serum Bactericidal Assay (SBA)
04:48
Results: Analysis of the Bacterial Assay
05:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个方案来测量血清中抗体的
志贺酸酯
活性。血清与细菌和外源补体混合, 孵育, 反应混合物被镀在琼脂板上。活细菌形成菌落, 这些菌落被计数, 使用自动菌落计数器, 并用于确定杀菌滴度。
Tags
Shigella
Bactericidal Assay
High-throughput
Antibody
Serum
Serial Dilutions
Target Bacteria
Complement
BRC
Plate Shaker
Incubation
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