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纳米等离子体增强散射和低放大显微镜成像对肿瘤衍生外质体的定量
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
DOI:

09:30 min

May 24, 2019

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Chapters

  • 00:04Title
  • 01:01Preparation of Nanoparticle Probes (AuNR-IgG)
  • 02:17Preparation of Extracellular Vesicle Capture Slides and Exosome Capture
  • 05:17Low-Magnification Dark-Field Microscope (LMDFM) Image Acquisition and Analysis
  • 07:48Results: Exosome Quantification by Analysis of LMDFM Images
  • 08:25Conclusion

Summary

Automatic Translation

由于缺乏快速准确的定量方法, 病细胞外衍生的病性和恶性细胞生物标志物的临床翻译受到阻碍。本报告描述了使用低放大暗场显微镜图像来量化小体积血清或血浆样本中的特定外源亚型。

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