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Bioengineering
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纳米等离子体增强散射和低放大显微镜成像对肿瘤衍生外质体的定量
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
Please note that all translations are automatically generated.
Click here for the English version.
纳米等离子体增强散射和低放大显微镜成像对肿瘤衍生外质体的定量
DOI:
10.3791/59177-v
•
09:30 min
•
May 24, 2019
•
Meihua Wan
2
,
Pouya Amrollahi
3
,
Dali Sun
,
Christopher Lyon
3
,
Tony Y. Hu
3
1
Department of Integrated Traditional Chinese and Western Medicine
,
West China Hospital of Sichuan University
,
2
Virginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute
,
Arizona State University
,
3
School of Biological and Health Systems Engineering
,
Arizona State University
,
4
Department of Electrical and Computer Engineering
,
North Dakota State University
Chapters
00:04
Title
01:01
Preparation of Nanoparticle Probes (AuNR-IgG)
02:17
Preparation of Extracellular Vesicle Capture Slides and Exosome Capture
05:17
Low-Magnification Dark-Field Microscope (LMDFM) Image Acquisition and Analysis
07:48
Results: Exosome Quantification by Analysis of LMDFM Images
08:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
由于缺乏快速准确的定量方法, 病细胞外衍生的病性和恶性细胞生物标志物的临床翻译受到阻碍。本报告描述了使用低放大暗场显微镜图像来量化小体积血清或血浆样本中的特定外源亚型。
Tags
Nanoplasmon-enhanced Scattering
NPES
Exosome Analysis
Exosome Purification
Biomarker Detection
Gold Nanorods
Antibody Conjugation
Exosome Capture
Low-magnification Microscopy
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