Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Abhijeet Patra; Tao Ding; Minghui Hong; Arthur Mark Richards; Ten It Wong; Xiaodong Zhou; Chester Lee Drum

doi:10.3791/55597

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

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09:23 min

December 13, 2017

DOI: 10.3791/55597-v

Abhijeet Patra¹, Tao Ding², Minghui Hong³, Arthur Mark Richards², Ten It Wong⁴, Xiaodong Zhou⁴, Chester Lee Drum²

¹NUS Nanoscience and Nanotechnology Initiative,National University of Singapore, ²Cardiovascular Research Institute, Yong Loo Lin School of Medicine,National University of Singapore, ³Department of Electrical and Computer Engineering,National University of Singapore, ⁴Institute of Materials Research Engineering,A*STAR (Agency for Science, Technology and Research)

Overview

This work describes a nanoimprinting lithography method to fabricate high-quality sensing arrays that operate on the principle of extraordinary optical transmission. The biosensor is designed for point-of-care applications, enabling the detection of cardiac troponin I in serum at clinically relevant concentrations.

Key Study Components

Area of Science

Neuroscience
Biotechnology
Biomedical Engineering

Background

The need for reliable biomarker detection in complex biological fluids.
Challenges associated with traditional biosensor technologies.
The principle of extraordinary optical transmission in biosensing.
Importance of point-of-care diagnostics in clinical settings.

Purpose of Study

To develop a low-cost, robust biosensor for cardiac troponin I detection.
To simplify the detection process without complex setups.
To demonstrate the feasibility of extraordinary transmission-based biosensors in clinical applications.

Methods Used

Nanoimprinting lithography for sensor fabrication.
Testing the biosensor in serum samples.
Animation representation of the fabrication process.
Evaluation of detection limits and performance metrics.

Main Results

The biosensor can detect cardiac troponin I at concentrations of 10-400 pg/mL.
It operates effectively in a point-of-care setting.
Demonstrated reliability in detecting biomarkers in complex fluids.
Eliminates the need for elaborate optical or electrical setups.

Conclusions

This method represents a significant advancement in biosensor technology.
It has the potential to improve point-of-care diagnostics for cardiac conditions.
Future work may focus on expanding the range of detectable biomarkers.

Frequently Asked Questions

What is the main application of the biosensor?

The biosensor is primarily used for detecting cardiac troponin I in serum samples.

How does the biosensor work?

It operates on the principle of extraordinary optical transmission, allowing for sensitive detection of biomarkers.

What are the advantages of this biosensor?

It is low-cost, robust, easy to use, and does not require complex setups.

What concentrations can the biosensor detect?

It can detect cardiac troponin I at clinically relevant concentrations ranging from 10 to 400 pg/mL.

Is the sensor fabrication process complex?

The fabrication is performed in a clean room, but the process is simplified for demonstration purposes.

Can this method be used for other biomarkers?

While this study focuses on cardiac troponin I, the method may be adapted for other biomarkers in future research.

This work describes a nanoimprinting lithography method to fabricate high-quality sensing arrays that work on the principle of extraordinary optical transmission. The biosensor is low-cost, robust, easy to use, and can detect cardiac troponin I in serum at clinically relevant concentrations (99^th percentile cutoff ∼10-400 pg/mL, depending on the assay).

The overall goal of this technique is to measure biomarkers present in a complex biological fluid background at clinically relevant concentrations in a point of care setting without the need for elaborate optical or electrical setups. This method can help in making biosensors based on extraordinary transmission a reality in point of care scenarios. The main advantage of this technique is that it doesn't need any complicated electrical or optical set-ups.

In our experiments it could reliably detect biomarkers at clinically relevant concentrations. Fabrication of the sensor chip is performed in the clean room and cannot be filmed. For demonstration purposes the procedure is represented here by an animation.

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