Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (<em>Camellia Sinensis </em>L.) Leaves

Weiting Jiao; Guoqin Ge; Rimao Hua; Jun Sun; Yeyun Li; Ruyan Hou

doi:10.3791/59312

/

Undersøgelse af metabolismen af seks systemiske insekticider i en nyetableret celle affjedrings kultur afledt af te (Camellia sinensis L.) Blade

JoVE Journal
Biochemistry
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JoVE Journal Biochemistry

Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves

Undersøgelse af metabolismen af seks systemiske insekticider i en nyetableret celle affjedrings kultur afledt af te (Camellia sinensis L.) Blade

DOI:

10.3791/59312-v

•

10:35 min

•

June 15, 2019

•

Weiting Jiao*^1,2, Guoqin Ge*¹, Rimao Hua, Jun Sun, Yeyun Li, Ruyan Hou

¹State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology,Anhui Province Key Lab of Analysis and Detection for Food Safety, ²School of Resource & Environment, Anhui Agricultural University,Key Laboratory of Agri-food Safety of Anhui Province

Chapters

00:00Title
01:46Tea Callus Culture
02:46Tea Cell Suspension Culture
03:43TTC Assay of Cell Viability
04:25Treatment and Sampling of Tea Cell Suspension Cultures with Insecticides
06:24Sample Preparation of Insecticides in Intact Tea Plant
06:56Instrument Analysis
07:54Results
10:02Conclusion

Summary

Automatic Translation

Dette arbejde præsenterer en protokol for etablering af en cellesuspension kultur afledt af te (Camellia sinensis L.) blade, der kan bruges til at studere metabolismen af eksterne forbindelser, der kan tages op af hele planten, såsom insekticider.

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