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使用前 Vivo 共聚焦显微镜可视化淋巴节点结构和细胞定位
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JoVE Journal Biology
Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy
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使用前 Vivo 共聚焦显微镜可视化淋巴节点结构和细胞定位

DOI:
10.3791/59335-v

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07:40 min

•

August 09, 2019

•

, , ,
1Ann Romney Center for Neurologic Diseases,Brigham and Women’s Hospital, Harvard Medical School, 2Center for Gastrointestinal Biology,Federal University of Minas Gerais

Chapters

  • 00:04Title
  • 00:56Antibody Cocktail Injection
  • 01:45Inguinal Draining Lymph Node (LN) Harvest
  • 02:49Popliteal Draining LN Harvest
  • 03:41LN Preparation and Ex-Vivo Confocal Microscopy Imaging
  • 05:11Results: Representative Whole LN Confocal Microscopy Imaging
  • 07:12Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一种在不改变器官结构的情况下在排空淋巴结中成像不同细胞群的技术。

Tags

Lymph NodeEx-vivoConfocal MicroscopyFluorescent AntibodiesMouse HandlingInguinal Lymph NodePopliteal Lymph NodeMicrosurgerySkin IncisionCell Viability

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