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Biochemistry
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用噻唑橙代替溴代或替代染料进行 DNA 电泳
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Biochemistry
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JoVE Journal
Biochemistry
DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
Please note that all translations are automatically generated.
Click here for the English version.
用噻唑橙代替溴代或替代染料进行 DNA 电泳
DOI:
10.3791/59341-v
•
04:18 min
•
March 31, 2019
•
Casey S. O’Neil
,
Jacie L. Beach
,
Todd D. Gruber
1
Department of Molecular Biology and Chemistry
,
Christopher Newport University
Chapters
00:04
Title
00:29
Preparing the Gel
01:29
Loading and Running the Gel
02:20
Visualization of Thiazole Orange Agarose Gel
03:08
Image Capture
03:28
Results: DNA Staining with Thiazole Orange in Electrophoresis Experiments
03:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议, 使用噻唑橙检测 DNA 的凝胶电泳实验。使用噻唑橙可以消除溴化乙酯, 并且可以使用紫外或蓝光进行荧光检测。
Tags
DNA Electrophoresis
Thiazole Orange
Ethidium Bromide
Alternative Dyes
UV Light
Blue Light
Agarose Gel
TAE Buffer
TBE Buffer
DNA Detection
DNA Visualization
DNA Extraction
Downstream Experiments
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