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评估细菌病原体免疫反应的声吞噬杀灭试验
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens
DOI:

08:47 min

April 05, 2019

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Chapters

  • 00:04Title
  • 01:21Culture, Differentiation, and Validation of HL-60 Cells
  • 02:30Preparation of Bacterial Stock Samples
  • 03:53Bacterial Treatment and Culture
  • 04:43HL-60 Bacterial Co-culture
  • 05:46Sample Plating and Overnight Incubation
  • 06:55Results: Determining Bacterial Cell Viability using OPKA
  • 07:55Conclusion

Summary

Automatic Translation

这种光吞噬杀灭方法用于比较吞噬免疫细胞根据不同的处理方法和条件对细菌做出反应和杀灭细菌的能力。传统上, 这种检测方法是评估针对视黄素细菌产生的抗体的效应功能的黄金标准。

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