JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
评估细菌病原体免疫反应的声吞噬杀灭试验
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens
Please note that all translations are automatically generated.
Click here for the English version.
评估细菌病原体免疫反应的声吞噬杀灭试验
DOI:
10.3791/59400-v
•
08:47 min
•
April 05, 2019
•
Amy V. Paschall
,
Dustin R. Middleton
,
Fikri Y. Avci
1
Department of Biochemistry and Molecular Biology, Center for Molecular Medicine and Complex Carbohydrate Research Center
,
University of Georgia
Chapters
00:04
Title
01:21
Culture, Differentiation, and Validation of HL-60 Cells
02:30
Preparation of Bacterial Stock Samples
03:53
Bacterial Treatment and Culture
04:43
HL-60 Bacterial Co-culture
05:46
Sample Plating and Overnight Incubation
06:55
Results: Determining Bacterial Cell Viability using OPKA
07:55
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这种光吞噬杀灭方法用于比较吞噬免疫细胞根据不同的处理方法和条件对细菌做出反应和杀灭细菌的能力。传统上, 这种检测方法是评估针对视黄素细菌产生的抗体的效应功能的黄金标准。
Tags
Opsonophagocytic Killing Assay
Immunotherapeutic
Antibacterial Activity
Phagocytosis
Streptococcus Pneumonia
HL60 Cells
Bacterial Stocks
Flow Cytometry
RPMI
Fetal Bovine Serum
Dimethyl Sulfoxide
DMF
Bacterial Strain
Glycerol
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
酶联免疫斑点法(ELISPOT):TH - 1细胞对微生物抗原的免疫应答的定量
抗原特异性
在体内
使用CFSE标记的靶细胞的杀伤含量
单克隆抗体的反对消极行政
H。 capsulatum
和其他真菌病原体
“发光法”的分类检测细菌病原体的记者噬菌体
斑马鱼的胚胎细胞内细菌病原体感染
饲养和注射<em>烟草天蛾</em>幼虫评估细菌的毒力
的视觉检测,以监测T6SS介导的细菌竞争
长期培养的干扰素γ酶联免疫检测方法的评估效应和记忆T细胞应答的牛应用
细菌病原体在细胞和有机水平宿主反应探针中的应用
一种高通
量志
贺氏菌特异性杀菌试验
Read Article